Written by Kristen Z. Swider, Capital Community College
Students in my microbiology class are relatively unfamiliar with the scientific concepts involved in the course and will often attempt to rely on memorization. However, due to the complex nature of the material, it is difficult to access information from the perspective of pure recall. As abstract concepts are discussed throughout a science course, many learners still operating in the concrete stage of development may be lost by a failure to attach understanding to anything of substance. As a result, the concepts are often missed during examinations. There is no requirement that an instructor complicate the approach in order to communicate scientific principles. For these reasons, the use of analogies to illustrate complex processes can enhance a student’s comprehension of the material and make connections that promote lifelong learning. Analogies may be presented to the learner as prepared elements of a lecture or they may be generated by the learners themselves. Self-generated analogies can and do occur spontaneously in discussion. Students are encouraged to develop and present analogies to the class. In either case, the interactive, social process of exploring analogies, whatever their source, contributes to the learning process.
How the ATP-binding Cassette (ABC) Transport System Works
- ATP-binding cassette (ABC) system: This involves substrate-specific binding proteins located in the bacterial periplasm, the gel-like substance between the bacterial cell wall and cytoplasmic membrane.
- The periplasmic-binding protein attaches temporarily to the substance to be transported and carries it to
- Meanwhile, ATP gets broken down into ADP, and phosphate, releasing energy. It is this energy that powers the transport of the substrate, by way of the membrane-binding transporter, across the membrane and into the cytoplasm.
- Examples of active transport by means of ABC systems include the transport of certain sugars and amino acids. There are hundreds of different ABC transport systems in bacteria.
The Bacterial Cell as an Entertainment Venue to Illustrate the ATP-Binding Cassette (ABC) Transport System
Bacterial cell: Entertainment Venue
Periplasm: Outer arena area
Substrate-specific binding protein: Event ticket
Cytoplasmic membrane: Inner arena barrier with turnstiles
Membrane-spanning transport protein: Turnstile
Cytoplasm: Event location (inner arena)
ATP: energy needed to move the turn-stile and allow entry of the substrate (Patron)
- The bacterial cell is the entertainment venue, with the cell wall being the outer boundary of the arena property. Once the patron reaches the arena, he/she can easily migrate through the cell wall to the inner arena (periplasm) since a “ticket” is not yet needed.
- In order for the patron to gain entry into the main arena area of the venue (cytoplasm), he/she must pick up a ticket at a will call/box office. Here in the periplasm, a patron will pick up a pre-prepared ticket (periplasmic binding protein) just before the entering the event.
- Before entering the main arena area, the patron with the ticket (transportable substance and periplasmic binding protein complex) must enter the arena through the turnstile (membrane-spanning transport protein). A turnstile is a form of gate which allows one person to pass at a time. A turnstile can restrict passage only to patrons who provide a coin or a ticket. It can also be made so as to enforce one-way traffic of people.
- Once at the turnstile, the ticket (periplasmic binding protein) gets left behind, and the transportable substrate (patron) can enter the cell via a turnstile.
- As the substrate (patron) moves through the turnstile, energy is required, and ATP is broken down. The patron (substrate) is now in the arena and can be used by the cell.